Restriction Enzymes
New England Biolabs, Inc. EnGen Spy dCas9 (SNAP-tag) – 400 pmol
EnGen Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity. EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
New England Biolabs, Inc. AvaI – 10000 units
A restriction endonuclease that recognizes the sequence C^YCGR_G.
New England Biolabs, Inc. MboII – 300 units
A restriction endonuclease that recognizes the sequence GAAGANNNNNNN_N^.
New England Biolabs, Inc. MboI – 2500 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. NEBuffer™ 1 – 5 ml
New England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity. This NEBuffer minus BSA formulation is available for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation (which is included with NEB restriction enzymes), select NEBuffer 1.1. New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1, NEBuffer 3.1 and CutSmart Buffer (NEB #B7204) are available.
New England Biolabs, Inc. Fpg – 500 units
Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3' and 5' to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
New England Biolabs, Inc. SNAP-Surface Alexa Fluor 488 – 50 nmol
SNAP-Surface Alexa Fluor 488 is a photostable green fluorescent substrate that can be used to label SNAP-tag fusion proteins in solution or on the surface of living cells.
New England Biolabs, Inc. HincII – 5000 units
A restriction endonuclease that recognizes the sequence GTY_^RAC.
New England Biolabs, Inc. FokI – 5000 units
A restriction endonuclease that recognizes the sequence GGATGNNNNNNNNN^NNNN_.
New England Biolabs, Inc. PciI – 1000 units
A restriction endonuclease that recognizes the sequence A^CATG_T.
New England Biolabs, Inc. RNase Inhibitor, Human Placenta – 2000 units
RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
New England Biolabs, Inc. BspQI HF-500 units
A Type IIS restriction endonuclease that recognizes the sequence GCTCTTC(1/4). An E. coli strain that carries the modified cloned BspQI gene from Bacillus sphaericus. BspQI-HF is an engineered and improved version of BspQI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)
New England Biolabs, Inc. PspXI – 1000 units
A restriction endonuclease that recognizes the sequence VC^TCGA_GB.